Construct: shRNA TRCN0000042999

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024628.4-1585s1c1
DNA Barcode:
CCTACAAGATAGCTTCCTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC12A8 (84561)
Gene Description:
solute carrier family 12 member 8
Transcript:
RefSeq NM_024628.4 (NON-CURRENT)
Match location:
Position 1585 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000042999)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACAAGATAGCTTCCTCTT
Hairpin Sequence:
5'-CCGG-CCTACAAGATAGCTTCCTCTT-CTCGAG-AAGAGGAAGCTATCTTGTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACAAGATAGCTTCCTCTTCTCGAGAAGAGGAAGCTATCTTGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACAAGATAGCTTCCTCTTCTCGAGAAGAGGAAGCTATCTTGTAGG-3'

Other clones with same target sequence:

(none)