Construct: shRNA TRCN0000043030

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199352.1-497s1c1
DNA Barcode:
GCTGCTACATTTAGCATCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC22A25 (387601)
Gene Description:
solute carrier family 22 member 25
Transcript:
RefSeq NM_199352.1 (NON-CURRENT)
Match location:
Position 497 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043030)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTACATTTAGCATCATT
Hairpin Sequence:
5'-CCGG-GCTGCTACATTTAGCATCATT-CTCGAG-AATGATGCTAAATGTAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTACATTTAGCATCATTCTCGAGAATGATGCTAAATGTAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTACATTTAGCATCATTCTCGAGAATGATGCTAAATGTAGCAGC-3'

Other clones with same target sequence:

(none)