Construct: shRNA TRCN0000043043

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015941.1-980s1c1
DNA Barcode:
CCTGGCATTCAGTCCTCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP6V1H (51606)
Gene Description:
ATPase H+ transporting V1 subunit H
Transcript:
RefSeq NM_015941.1 (NON-CURRENT)
Match location:
Position 980 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043043)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGCATTCAGTCCTCAAAT
Hairpin Sequence:
5'-CCGG-CCTGGCATTCAGTCCTCAAAT-CTCGAG-ATTTGAGGACTGAATGCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGCATTCAGTCCTCAAATCTCGAGATTTGAGGACTGAATGCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGCATTCAGTCCTCAAATCTCGAGATTTGAGGACTGAATGCCAGG-3'

Other clones with same target sequence:

(none)