Construct: shRNA TRCN0000043057

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005415.3-2302s1c1
DNA Barcode:
CCATCAGTACAACACATTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC20A1 (6574)
Gene Description:
solute carrier family 20 member 1
Transcript:
RefSeq NM_005415.3 (NON-CURRENT)
Match location:
Position 2302 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043057)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCAGTACAACACATTGTA
Hairpin Sequence:
5'-CCGG-CCATCAGTACAACACATTGTA-CTCGAG-TACAATGTGTTGTACTGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCAGTACAACACATTGTACTCGAGTACAATGTGTTGTACTGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCAGTACAACACATTGTACTCGAGTACAATGTGTTGTACTGATGG-3'

Other clones with same target sequence:

  1. TRCN0000289801