Construct: shRNA TRCN0000043064

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005630.1-1531s1c1
DNA Barcode:
CCTCCAAGCAACTGATCTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLCO2A1 (6578)
Gene Description:
solute carrier organic anion transporter family member 2A1
Transcript:
RefSeq NM_005630.1 (NON-CURRENT)
Match location:
Position 1531 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043064)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCAAGCAACTGATCTATT
Hairpin Sequence:
5'-CCGG-CCTCCAAGCAACTGATCTATT-CTCGAG-AATAGATCAGTTGCTTGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCAAGCAACTGATCTATTCTCGAGAATAGATCAGTTGCTTGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCAAGCAACTGATCTATTCTCGAGAATAGATCAGTTGCTTGGAGG-3'

Other clones with same target sequence:

(none)