Construct: shRNA TRCN0000043074

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006749.3-414s1c1
DNA Barcode:
CATAGCTTTCATCTTGGCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC20A2 (6575)
Gene Description:
solute carrier family 20 member 2
Transcript:
RefSeq NM_006749.3 (NON-CURRENT)
Match location:
Position 414 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043074)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATAGCTTTCATCTTGGCCTT
Hairpin Sequence:
5'-CCGG-CATAGCTTTCATCTTGGCCTT-CTCGAG-AAGGCCAAGATGAAAGCTATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATAGCTTTCATCTTGGCCTTCTCGAGAAGGCCAAGATGAAAGCTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATAGCTTTCATCTTGGCCTTCTCGAGAAGGCCAAGATGAAAGCTATG-3'

Other clones with same target sequence:

(none)