Construct: shRNA TRCN0000043128

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003056.2-1840s1c1
DNA Barcode:
CCAGTTATACTCCGTGTACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC19A1 (6573)
Gene Description:
solute carrier family 19 member 1
Transcript:
RefSeq NM_003056.2 (NON-CURRENT)
Match location:
Position 1840 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043128)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTTATACTCCGTGTACTT
Hairpin Sequence:
5'-CCGG-CCAGTTATACTCCGTGTACTT-CTCGAG-AAGTACACGGAGTATAACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTTATACTCCGTGTACTTCTCGAGAAGTACACGGAGTATAACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTTATACTCCGTGTACTTCTCGAGAAGTACACGGAGTATAACTGG-3'

Other clones with same target sequence:

(none)