Construct: shRNA TRCN0000043157

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001676.2-2840s1c1
DNA Barcode:
CTTGTGTATTTCACCGTCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP12A (479)
Gene Description:
ATPase H+/K+ transporting non-gastric alpha2 subunit
Transcript:
RefSeq NM_001676.2 (NON-CURRENT)
Match location:
Position 2840 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043157)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGTGTATTTCACCGTCTAT
Hairpin Sequence:
5'-CCGG-CTTGTGTATTTCACCGTCTAT-CTCGAG-ATAGACGGTGAAATACACAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGTGTATTTCACCGTCTATCTCGAGATAGACGGTGAAATACACAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGTGTATTTCACCGTCTATCTCGAGATAGACGGTGAAATACACAAG-3'

Other clones with same target sequence:

(none)