Construct: shRNA TRCN0000043159

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003615.2-2395s1c1
DNA Barcode:
GCAATGAAACTCTAGCACAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC4A7 (9497)
Gene Description:
solute carrier family 4 member 7
Transcript:
RefSeq NM_003615.2 (NON-CURRENT)
Match location:
Position 2395 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043159)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATGAAACTCTAGCACAAT
Hairpin Sequence:
5'-CCGG-GCAATGAAACTCTAGCACAAT-CTCGAG-ATTGTGCTAGAGTTTCATTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATGAAACTCTAGCACAATCTCGAGATTGTGCTAGAGTTTCATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATGAAACTCTAGCACAATCTCGAGATTGTGCTAGAGTTTCATTGC-3'

Other clones with same target sequence:

(none)