Construct: shRNA TRCN0000043212

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003057.2-1383s1c1
DNA Barcode:
CCTGCACTGGTTAAACATCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC22A1 (6580)
Gene Description:
solute carrier family 22 member 1
Transcript:
RefSeq NM_003057.2 (NON-CURRENT)
Match location:
Position 1383 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043212)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCACTGGTTAAACATCAT
Hairpin Sequence:
5'-CCGG-CCTGCACTGGTTAAACATCAT-CTCGAG-ATGATGTTTAACCAGTGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCACTGGTTAAACATCATCTCGAGATGATGTTTAACCAGTGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCACTGGTTAAACATCATCTCGAGATGATGTTTAACCAGTGCAGG-3'

Other clones with same target sequence:

(none)