Construct: shRNA TRCN0000043222

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016526.3-212s1c1
DNA Barcode:
CGGTACCTGGATGGCATGGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BET1L (51272)
Gene Description:
Bet1 golgi vesicular membrane trafficking protein like
Transcript:
RefSeq NM_016526.3 (NON-CURRENT)
Match location:
Position 212 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043222)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTACCTGGATGGCATGGTA
Hairpin Sequence:
5'-CCGG-CGGTACCTGGATGGCATGGTA-CTCGAG-TACCATGCCATCCAGGTACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTACCTGGATGGCATGGTACTCGAGTACCATGCCATCCAGGTACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTACCTGGATGGCATGGTACTCGAGTACCATGCCATCCAGGTACCG-3'

Other clones with same target sequence:

(none)