Construct: shRNA TRCN0000043275

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019844.1-673s1c1
DNA Barcode:
GTAGTTTGAATGCAATAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLCO1B3 (28234)
Gene Description:
solute carrier organic anion transporter family member 1B3
Transcript:
RefSeq NM_019844.1 (NON-CURRENT)
Match location:
Position 673 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043275)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGTTTGAATGCAATAGGAA
Hairpin Sequence:
5'-CCGG-GTAGTTTGAATGCAATAGGAA-CTCGAG-TTCCTATTGCATTCAAACTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGTTTGAATGCAATAGGAACTCGAGTTCCTATTGCATTCAAACTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGTTTGAATGCAATAGGAACTCGAGTTCCTATTGCATTCAAACTAC-3'

Other clones with same target sequence:

(none)