Construct: shRNA TRCN0000043350

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001859.2-408s1c1
DNA Barcode:
GCGTAAGTCACAAGTCAGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC31A1 (1317)
Gene Description:
solute carrier family 31 member 1
Transcript:
RefSeq NM_001859.2 (NON-CURRENT)
Match location:
Position 408 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043350)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTAAGTCACAAGTCAGCAT
Hairpin Sequence:
5'-CCGG-GCGTAAGTCACAAGTCAGCAT-CTCGAG-ATGCTGACTTGTGACTTACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTAAGTCACAAGTCAGCATCTCGAGATGCTGACTTGTGACTTACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTAAGTCACAAGTCAGCATCTCGAGATGCTGACTTGTGACTTACGC-3'

Other clones with same target sequence:

  1. TRCN0000300462