Construct: shRNA TRCN0000043361

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145206.1-542s1c1
DNA Barcode:
CGAGCAAATTGGTCAGGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VTI1A (143187)
Gene Description:
vesicle transport through interaction with t-SNAREs 1A
Transcript:
RefSeq NM_145206.1 (NON-CURRENT)
Match location:
Position 542 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043361)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGCAAATTGGTCAGGAGAT
Hairpin Sequence:
5'-CCGG-CGAGCAAATTGGTCAGGAGAT-CTCGAG-ATCTCCTGACCAATTTGCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGCAAATTGGTCAGGAGATCTCGAGATCTCCTGACCAATTTGCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGCAAATTGGTCAGGAGATCTCGAGATCTCCTGACCAATTTGCTCG-3'

Other clones with same target sequence:

(none)