Construct: shRNA TRCN0000043369

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001679.1-310s1c1
DNA Barcode:
GCAGGGTACATTGAAGACCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP1B3 (483)
Gene Description:
ATPase Na+/K+ transporting subunit beta 3
Transcript:
RefSeq NM_001679.1 (NON-CURRENT)
Match location:
Position 310 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043369)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGGTACATTGAAGACCTT
Hairpin Sequence:
5'-CCGG-GCAGGGTACATTGAAGACCTT-CTCGAG-AAGGTCTTCAATGTACCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGGTACATTGAAGACCTTCTCGAGAAGGTCTTCAATGTACCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGGTACATTGAAGACCTTCTCGAGAAGGTCTTCAATGTACCCTGC-3'

Other clones with same target sequence:

(none)