Construct: shRNA TRCN0000043390

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003645.2-1011s1c1
DNA Barcode:
GCTGCACTACTGATTGGCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC27A2 (11001)
Gene Description:
solute carrier family 27 member 2
Transcript:
RefSeq NM_003645.2 (NON-CURRENT)
Match location:
Position 1011 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043390)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCACTACTGATTGGCATT
Hairpin Sequence:
5'-CCGG-GCTGCACTACTGATTGGCATT-CTCGAG-AATGCCAATCAGTAGTGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCACTACTGATTGGCATTCTCGAGAATGCCAATCAGTAGTGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCACTACTGATTGGCATTCTCGAGAATGCCAATCAGTAGTGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000310629