Construct: shRNA TRCN0000043492

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005176.3-369s1c1
DNA Barcode:
AGGAACCCTTCTCTGAAGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP5MC2 (517)
Gene Description:
ATP synthase membrane subunit c locus 2
Transcript:
RefSeq NM_005176.3 (NON-CURRENT)
Match location:
Position 369 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043492)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAACCCTTCTCTGAAGCAA
Hairpin Sequence:
5'-CCGG-AGGAACCCTTCTCTGAAGCAA-CTCGAG-TTGCTTCAGAGAAGGGTTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAACCCTTCTCTGAAGCAACTCGAGTTGCTTCAGAGAAGGGTTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAACCCTTCTCTGAAGCAACTCGAGTTGCTTCAGAGAAGGGTTCCT-3'

Other clones with same target sequence:

(none)