Construct: shRNA TRCN0000043494

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012463.2-377s1c1
DNA Barcode:
CGTAAGTTCTTTCCAAAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP6V0A2 (23545)
Gene Description:
ATPase H+ transporting V0 subunit a2
Transcript:
RefSeq NM_012463.2 (NON-CURRENT)
Match location:
Position 377 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043494)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTAAGTTCTTTCCAAAGAAA
Hairpin Sequence:
5'-CCGG-CGTAAGTTCTTTCCAAAGAAA-CTCGAG-TTTCTTTGGAAAGAACTTACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTAAGTTCTTTCCAAAGAAACTCGAGTTTCTTTGGAAAGAACTTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTAAGTTCTTTCCAAAGAAACTCGAGTTTCTTTGGAAAGAACTTACG-3'

Other clones with same target sequence:

(none)