Construct: shRNA TRCN0000043501

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016529.3-2526s1c1
DNA Barcode:
GCTTGGACTAAATTCAGTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP8A2 (51761)
Gene Description:
ATPase phospholipid transporting 8A2
Transcript:
RefSeq NM_016529.3 (NON-CURRENT)
Match location:
Position 2526 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043501)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGGACTAAATTCAGTCAT
Hairpin Sequence:
5'-CCGG-GCTTGGACTAAATTCAGTCAT-CTCGAG-ATGACTGAATTTAGTCCAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGGACTAAATTCAGTCATCTCGAGATGACTGAATTTAGTCCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGGACTAAATTCAGTCATCTCGAGATGACTGAATTTAGTCCAAGC-3'

Other clones with same target sequence:

(none)