Construct: shRNA TRCN0000043624

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012434.3-199s1c1
DNA Barcode:
GCTCGTTACAACTTAGCAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC17A5 (26503)
Gene Description:
solute carrier family 17 member 5
Transcript:
RefSeq NM_012434.3 (NON-CURRENT)
Match location:
Position 199 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043624)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGTTACAACTTAGCAATT
Hairpin Sequence:
5'-CCGG-GCTCGTTACAACTTAGCAATT-CTCGAG-AATTGCTAAGTTGTAACGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGTTACAACTTAGCAATTCTCGAGAATTGCTAAGTTGTAACGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGTTACAACTTAGCAATTCTCGAGAATTGCTAAGTTGTAACGAGC-3'

Other clones with same target sequence:

  1. TRCN0000300186