Construct: shRNA TRCN0000043679

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018344.3-185s1c1
DNA Barcode:
CTGTGGCACATACATCATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC29A3 (55315)
Gene Description:
solute carrier family 29 member 3
Transcript:
RefSeq NM_018344.3 (NON-CURRENT)
Match location:
Position 185 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043679)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTGGCACATACATCATCTT
Hairpin Sequence:
5'-CCGG-CTGTGGCACATACATCATCTT-CTCGAG-AAGATGATGTATGTGCCACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTGGCACATACATCATCTTCTCGAGAAGATGATGTATGTGCCACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTGGCACATACATCATCTTCTCGAGAAGATGATGTATGTGCCACAG-3'

Other clones with same target sequence:

(none)