Construct: shRNA TRCN0000043719

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005183.1-4796s1c1
DNA Barcode:
CCAGGACTATTTCCGCAAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CACNA1F (778)
Gene Description:
calcium voltage-gated channel subunit alpha1 F
Transcript:
RefSeq NM_005183.1 (NON-CURRENT)
Match location:
Position 4796 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043719)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGACTATTTCCGCAAATT
Hairpin Sequence:
5'-CCGG-CCAGGACTATTTCCGCAAATT-CTCGAG-AATTTGCGGAAATAGTCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGACTATTTCCGCAAATTCTCGAGAATTTGCGGAAATAGTCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGACTATTTCCGCAAATTCTCGAGAATTTGCGGAAATAGTCCTGG-3'

Other clones with same target sequence:

(none)