Construct: shRNA TRCN0000043726

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014217.1-1167s1c1
DNA Barcode:
CTGAAGACTGAGAGTATCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNK2 (3776)
Gene Description:
potassium two pore domain channel subfamily K member 2
Transcript:
RefSeq NM_014217.1 (NON-CURRENT)
Match location:
Position 1167 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043726)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGAAGACTGAGAGTATCTAT
Hairpin Sequence:
5'-CCGG-CTGAAGACTGAGAGTATCTAT-CTCGAG-ATAGATACTCTCAGTCTTCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGAAGACTGAGAGTATCTATCTCGAGATAGATACTCTCAGTCTTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGAAGACTGAGAGTATCTATCTCGAGATAGATACTCTCAGTCTTCAG-3'

Other clones with same target sequence:

(none)