Construct: shRNA TRCN0000043750

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002246.1-323s1c1
DNA Barcode:
GCTGCGCCTCAAGCCGCACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNK3 (3777)
Gene Description:
potassium two pore domain channel subfamily K member 3
Transcript:
RefSeq NM_002246.1 (NON-CURRENT)
Match location:
Position 323 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043750)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCGCCTCAAGCCGCACAA
Hairpin Sequence:
5'-CCGG-GCTGCGCCTCAAGCCGCACAA-CTCGAG-TTGTGCGGCTTGAGGCGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCGCCTCAAGCCGCACAACTCGAGTTGTGCGGCTTGAGGCGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCGCCTCAAGCCGCACAACTCGAGTTGTGCGGCTTGAGGCGCAGC-3'

Other clones with same target sequence:

(none)