Construct: shRNA TRCN0000043767

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006359.1-1883s1c1
DNA Barcode:
CCCAAGGAGATTTATGGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC9A6 (10479)
Gene Description:
solute carrier family 9 member A6
Transcript:
RefSeq NM_006359.1 (NON-CURRENT)
Match location:
Position 1883 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043767)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAGGAGATTTATGGGAAA
Hairpin Sequence:
5'-CCGG-CCCAAGGAGATTTATGGGAAA-CTCGAG-TTTCCCATAAATCTCCTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAGGAGATTTATGGGAAACTCGAGTTTCCCATAAATCTCCTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAGGAGATTTATGGGAAACTCGAGTTTCCCATAAATCTCCTTGGG-3'

Other clones with same target sequence:

(none)