Construct: shRNA TRCN0000043772

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000722.1-3237s1c1
DNA Barcode:
CCCTGTGGTATATCATTGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CACNA2D1 (781)
Gene Description:
calcium voltage-gated channel auxiliary subunit alpha2delta 1
Transcript:
RefSeq NM_000722.1 (NON-CURRENT)
Match location:
Position 3237 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043772)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGTGGTATATCATTGGAA
Hairpin Sequence:
5'-CCGG-CCCTGTGGTATATCATTGGAA-CTCGAG-TTCCAATGATATACCACAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGTGGTATATCATTGGAACTCGAGTTCCAATGATATACCACAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGTGGTATATCATTGGAACTCGAGTTCCAATGATATACCACAGGG-3'

Other clones with same target sequence:

(none)