Construct: shRNA TRCN0000043846

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021614.2-1238s1c1
DNA Barcode:
CCAATGTTCTTAAGACTCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNN2 (3781)
Gene Description:
potassium calcium-activated channel subfamily N member 2
Transcript:
RefSeq NM_021614.2 (NON-CURRENT)
Match location:
Position 1238 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043846)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAATGTTCTTAAGACTCTAT
Hairpin Sequence:
5'-CCGG-CCAATGTTCTTAAGACTCTAT-CTCGAG-ATAGAGTCTTAAGAACATTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAATGTTCTTAAGACTCTATCTCGAGATAGAGTCTTAAGAACATTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAATGTTCTTAAGACTCTATCTCGAGATAGAGTCTTAAGAACATTGG-3'

Other clones with same target sequence:

(none)