Construct: shRNA TRCN0000043895

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002249.3-2500s1c1
DNA Barcode:
CGTACACAAGTTCAAGCAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNN3 (3782)
Gene Description:
potassium calcium-activated channel subfamily N member 3
Transcript:
RefSeq NM_002249.3 (NON-CURRENT)
Match location:
Position 2500 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043895)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTACACAAGTTCAAGCAGTT
Hairpin Sequence:
5'-CCGG-CGTACACAAGTTCAAGCAGTT-CTCGAG-AACTGCTTGAACTTGTGTACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTACACAAGTTCAAGCAGTTCTCGAGAACTGCTTGAACTTGTGTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTACACAAGTTCAAGCAGTTCTCGAGAACTGCTTGAACTTGTGTACG-3'

Other clones with same target sequence:

(none)