Construct: shRNA TRCN0000043927

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031212.2-1352s1c1
DNA Barcode:
CTTGGCTTTGAACTCACACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC25A28 (81894)
Gene Description:
solute carrier family 25 member 28
Transcript:
RefSeq NM_031212.2 (NON-CURRENT)
Match location:
Position 1352 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043927)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGGCTTTGAACTCACACAT
Hairpin Sequence:
5'-CCGG-CTTGGCTTTGAACTCACACAT-CTCGAG-ATGTGTGAGTTCAAAGCCAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGGCTTTGAACTCACACATCTCGAGATGTGTGAGTTCAAAGCCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGGCTTTGAACTCACACATCTCGAGATGTGTGAGTTCAAAGCCAAG-3'

Other clones with same target sequence:

(none)