Construct: shRNA TRCN0000043928

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000336.1-1009s1c1
DNA Barcode:
CCTGAAGTTGATCCTGGACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCNN1B (6338)
Gene Description:
sodium channel epithelial 1 beta subunit
Transcript:
RefSeq NM_000336.1 (NON-CURRENT)
Match location:
Position 1009 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000043928)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAGTTGATCCTGGACAT
Hairpin Sequence:
5'-CCGG-CCTGAAGTTGATCCTGGACAT-CTCGAG-ATGTCCAGGATCAACTTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAGTTGATCCTGGACATCTCGAGATGTCCAGGATCAACTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAGTTGATCCTGGACATCTCGAGATGTCCAGGATCAACTTCAGG-3'

Other clones with same target sequence:

(none)