Construct: shRNA TRCN0000044030

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003305.1-1662s1c1
DNA Barcode:
CGCTGCCCAATATCACAGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRPC3 (7222)
Gene Description:
transient receptor potential cation channel subfamily C member 3
Transcript:
RefSeq NM_003305.1 (NON-CURRENT)
Match location:
Position 1662 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044030)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTGCCCAATATCACAGTTA
Hairpin Sequence:
5'-CCGG-CGCTGCCCAATATCACAGTTA-CTCGAG-TAACTGTGATATTGGGCAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTGCCCAATATCACAGTTACTCGAGTAACTGTGATATTGGGCAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTGCCCAATATCACAGTTACTCGAGTAACTGTGATATTGGGCAGCG-3'

Other clones with same target sequence:

(none)