Construct: shRNA TRCN0000044161

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005140.1-1253s1c1
DNA Barcode:
CCCTGTAAAGGATGAGGAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CNGA2 (1260)
Gene Description:
cyclic nucleotide gated channel subunit alpha 2
Transcript:
RefSeq NM_005140.1 (CURRENT)
Match location:
Position 1253 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044161)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGTAAAGGATGAGGAGTA
Hairpin Sequence:
5'-CCGG-CCCTGTAAAGGATGAGGAGTA-CTCGAG-TACTCCTCATCCTTTACAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGTAAAGGATGAGGAGTACTCGAGTACTCCTCATCCTTTACAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGTAAAGGATGAGGAGTACTCGAGTACTCCTCATCCTTTACAGGG-3'

Other clones with same target sequence:

(none)