Construct: shRNA TRCN0000044181

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030631.1-598s1c1
DNA Barcode:
GTACAAGAAATTGCTGGGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC25A21 (89874)
Gene Description:
solute carrier family 25 member 21
Transcript:
RefSeq NM_030631.1 (NON-CURRENT)
Match location:
Position 598 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044181)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACAAGAAATTGCTGGGATA
Hairpin Sequence:
5'-CCGG-GTACAAGAAATTGCTGGGATA-CTCGAG-TATCCCAGCAATTTCTTGTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACAAGAAATTGCTGGGATACTCGAGTATCCCAGCAATTTCTTGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACAAGAAATTGCTGGGATACTCGAGTATCCCAGCAATTTCTTGTAC-3'

Other clones with same target sequence:

(none)