Construct: shRNA TRCN0000044200

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001298.1-571s1c1
DNA Barcode:
CCTGTCTTCTATAACTGGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CNGA3 (1261)
Gene Description:
cyclic nucleotide gated channel subunit alpha 3
Transcript:
RefSeq NM_001298.1 (NON-CURRENT)
Match location:
Position 571 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044200)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTCTTCTATAACTGGTAT
Hairpin Sequence:
5'-CCGG-CCTGTCTTCTATAACTGGTAT-CTCGAG-ATACCAGTTATAGAAGACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTCTTCTATAACTGGTATCTCGAGATACCAGTTATAGAAGACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTCTTCTATAACTGGTATCTCGAGATACCAGTTATAGAAGACAGG-3'

Other clones with same target sequence:

(none)