Construct: shRNA TRCN0000044210

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021098.1-4229s1c1
DNA Barcode:
CCTCAGCGTCTCCAATTACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CACNA1H (8912)
Gene Description:
calcium voltage-gated channel subunit alpha1 H
Transcript:
RefSeq NM_021098.1 (NON-CURRENT)
Match location:
Position 4229 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044210)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAGCGTCTCCAATTACAT
Hairpin Sequence:
5'-CCGG-CCTCAGCGTCTCCAATTACAT-CTCGAG-ATGTAATTGGAGACGCTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAGCGTCTCCAATTACATCTCGAGATGTAATTGGAGACGCTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAGCGTCTCCAATTACATCTCGAGATGTAATTGGAGACGCTGAGG-3'

Other clones with same target sequence:

  1. TRCN0000331243