Construct: shRNA TRCN0000044228

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019098.2-772s1c1
DNA Barcode:
CCCATATCAAACCGCAGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CNGB3 (54714)
Gene Description:
cyclic nucleotide gated channel subunit beta 3
Transcript:
RefSeq NM_019098.2 (NON-CURRENT)
Match location:
Position 772 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044228)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATATCAAACCGCAGACAA
Hairpin Sequence:
5'-CCGG-CCCATATCAAACCGCAGACAA-CTCGAG-TTGTCTGCGGTTTGATATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATATCAAACCGCAGACAACTCGAGTTGTCTGCGGTTTGATATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATATCAAACCGCAGACAACTCGAGTTGTCTGCGGTTTGATATGGG-3'

Other clones with same target sequence:

(none)