Construct: shRNA TRCN0000044256

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016289.2-439s1c1
DNA Barcode:
GCTACAGAAGAAGTTTCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAB39 (51719)
Gene Description:
calcium binding protein 39
Transcript:
RefSeq NM_016289.2 (NON-CURRENT)
Match location:
Position 439 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044256)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACAGAAGAAGTTTCCAAA
Hairpin Sequence:
5'-CCGG-GCTACAGAAGAAGTTTCCAAA-CTCGAG-TTTGGAAACTTCTTCTGTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACAGAAGAAGTTTCCAAACTCGAGTTTGGAAACTTCTTCTGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACAGAAGAAGTTTCCAAACTCGAGTTTGGAAACTTCTTCTGTAGC-3'

Other clones with same target sequence:

  1. TRCN0000290761