Construct: shRNA TRCN0000044272

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031895.4-616s1c1
DNA Barcode:
GAGCAACATCATCGGCGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CACNG8 (59283)
Gene Description:
calcium voltage-gated channel auxiliary subunit gamma 8
Transcript:
RefSeq NM_031895.4 (NON-CURRENT)
Match location:
Position 616 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044272)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCAACATCATCGGCGTGAT
Hairpin Sequence:
5'-CCGG-GAGCAACATCATCGGCGTGAT-CTCGAG-ATCACGCCGATGATGTTGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCAACATCATCGGCGTGATCTCGAGATCACGCCGATGATGTTGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCAACATCATCGGCGTGATCTCGAGATCACGCCGATGATGTTGCTC-3'

Other clones with same target sequence:

(none)