Construct: shRNA TRCN0000044307

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012283.1-1074s1c1
DNA Barcode:
CAGCTATTGGTGGGCCGTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNG2 (26251)
Gene Description:
potassium voltage-gated channel modifier subfamily G member 2
Transcript:
RefSeq NM_012283.1 (CURRENT)
Match location:
Position 1074 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044307)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCTATTGGTGGGCCGTCAT
Hairpin Sequence:
5'-CCGG-CAGCTATTGGTGGGCCGTCAT-CTCGAG-ATGACGGCCCACCAATAGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCTATTGGTGGGCCGTCATCTCGAGATGACGGCCCACCAATAGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCTATTGGTGGGCCGTCATCTCGAGATGACGGCCCACCAATAGCTG-3'

Other clones with same target sequence:

(none)