Construct: shRNA TRCN0000044312

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006920.2-3010s1c1
DNA Barcode:
GCTGTGGATAGGATGCACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCN1A (6323)
Gene Description:
sodium voltage-gated channel alpha subunit 1
Transcript:
RefSeq NM_006920.2 (NON-CURRENT)
Match location:
Position 3010 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044312)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGGATAGGATGCACAAA
Hairpin Sequence:
5'-CCGG-GCTGTGGATAGGATGCACAAA-CTCGAG-TTTGTGCATCCTATCCACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGGATAGGATGCACAAACTCGAGTTTGTGCATCCTATCCACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGGATAGGATGCACAAACTCGAGTTTGTGCATCCTATCCACAGC-3'

Other clones with same target sequence:

(none)