Construct: shRNA TRCN0000044331

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002239.2-996s1c1
DNA Barcode:
CCTCACAATTTGCCACGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNJ3 (3760)
Gene Description:
potassium inwardly rectifying channel subfamily J member 3
Transcript:
RefSeq NM_002239.2 (NON-CURRENT)
Match location:
Position 996 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044331)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCACAATTTGCCACGTGAT
Hairpin Sequence:
5'-CCGG-CCTCACAATTTGCCACGTGAT-CTCGAG-ATCACGTGGCAAATTGTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCACAATTTGCCACGTGATCTCGAGATCACGTGGCAAATTGTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCACAATTTGCCACGTGATCTCGAGATCACGTGGCAAATTGTGAGG-3'

Other clones with same target sequence:

(none)