Construct: shRNA TRCN0000044358

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013943.1-4128s1c1
DNA Barcode:
GCCGTAATGTTGAACAGAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLIC4 (25932)
Gene Description:
chloride intracellular channel 4
Transcript:
RefSeq NM_013943.1 (NON-CURRENT)
Match location:
Position 4128 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044358)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGTAATGTTGAACAGAATT
Hairpin Sequence:
5'-CCGG-GCCGTAATGTTGAACAGAATT-CTCGAG-AATTCTGTTCAACATTACGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGTAATGTTGAACAGAATTCTCGAGAATTCTGTTCAACATTACGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGTAATGTTGAACAGAATTCTCGAGAATTCTGTTCAACATTACGGC-3'

Other clones with same target sequence:

  1. TRCN0000300975