Construct: shRNA TRCN0000044370

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014407.3-1295s1c1
DNA Barcode:
GCATCAGTTCAAACTGTGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNMB3 (27094)
Gene Description:
potassium calcium-activated channel subfamily M regulatory beta subunit 3
Transcript:
RefSeq NM_014407.3 (CURRENT)
Match location:
Position 1295 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044370)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCAGTTCAAACTGTGCAT
Hairpin Sequence:
5'-CCGG-GCATCAGTTCAAACTGTGCAT-CTCGAG-ATGCACAGTTTGAACTGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCAGTTCAAACTGTGCATCTCGAGATGCACAGTTTGAACTGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCAGTTCAAACTGTGCATCTCGAGATGCACAGTTTGAACTGATGC-3'

Other clones with same target sequence:

(none)