Construct: shRNA TRCN0000044382

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016929.1-892s1c1
DNA Barcode:
GCCAAGAAATACCGCAACTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLIC5 (53405)
Gene Description:
chloride intracellular channel 5
Transcript:
RefSeq NM_016929.1 (NON-CURRENT)
Match location:
Position 892 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044382)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGAAATACCGCAACTAT
Hairpin Sequence:
5'-CCGG-GCCAAGAAATACCGCAACTAT-CTCGAG-ATAGTTGCGGTATTTCTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGAAATACCGCAACTATCTCGAGATAGTTGCGGTATTTCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGAAATACCGCAACTATCTCGAGATAGTTGCGGTATTTCTTGGC-3'

Other clones with same target sequence:

(none)