Construct: shRNA TRCN0000044428

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004982.2-2082s1c1
DNA Barcode:
CCATGATAACTGCATTTGGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNJ8 (3764)
Gene Description:
potassium inwardly rectifying channel subfamily J member 8
Transcript:
RefSeq NM_004982.2 (NON-CURRENT)
Match location:
Position 2082 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044428)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGATAACTGCATTTGGTA
Hairpin Sequence:
5'-CCGG-CCATGATAACTGCATTTGGTA-CTCGAG-TACCAAATGCAGTTATCATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGATAACTGCATTTGGTACTCGAGTACCAAATGCAGTTATCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGATAACTGCATTTGGTACTCGAGTACCAAATGCAGTTATCATGG-3'

Other clones with same target sequence:

(none)