Construct: shRNA TRCN0000044470

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002976.1-1552s1c1
DNA Barcode:
CCCATTATACTGGTATAAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCN7A (6332)
Gene Description:
sodium voltage-gated channel alpha subunit 7
Transcript:
RefSeq NM_002976.1 (NON-CURRENT)
Match location:
Position 1552 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044470)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATTATACTGGTATAAGTT
Hairpin Sequence:
5'-CCGG-CCCATTATACTGGTATAAGTT-CTCGAG-AACTTATACCAGTATAATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATTATACTGGTATAAGTTCTCGAGAACTTATACCAGTATAATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATTATACTGGTATAAGTTCTCGAGAACTTATACCAGTATAATGGG-3'

Other clones with same target sequence:

(none)