Construct: shRNA TRCN0000044491

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014191.1-4468s1c1
DNA Barcode:
GCAGCCTAAGTATGAGGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCN8A (6334)
Gene Description:
sodium voltage-gated channel alpha subunit 8
Transcript:
RefSeq NM_014191.1 (NON-CURRENT)
Match location:
Position 4468 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044491)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCCTAAGTATGAGGACAA
Hairpin Sequence:
5'-CCGG-GCAGCCTAAGTATGAGGACAA-CTCGAG-TTGTCCTCATACTTAGGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCCTAAGTATGAGGACAACTCGAGTTGTCCTCATACTTAGGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCCTAAGTATGAGGACAACTCGAGTTGTCCTCATACTTAGGCTGC-3'

Other clones with same target sequence:

(none)