Construct: shRNA TRCN0000044575

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013348.2-1621s1c1
DNA Barcode:
GATGAGACTGAGGAAGGGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNJ14 (3770)
Gene Description:
potassium inwardly rectifying channel subfamily J member 14
Transcript:
RefSeq NM_013348.2 (NON-CURRENT)
Match location:
Position 1621 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044575)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGAGACTGAGGAAGGGAAT
Hairpin Sequence:
5'-CCGG-GATGAGACTGAGGAAGGGAAT-CTCGAG-ATTCCCTTCCTCAGTCTCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGAGACTGAGGAAGGGAATCTCGAGATTCCCTTCCTCAGTCTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGAGACTGAGGAAGGGAATCTCGAGATTCCCTTCCTCAGTCTCATC-3'

Other clones with same target sequence:

(none)