Construct: shRNA TRCN0000044611

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002243.3-1332s1c1
DNA Barcode:
GCAGATTCTGAGAAACAGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNJ15 (3772)
Gene Description:
potassium inwardly rectifying channel subfamily J member 15
Transcript:
RefSeq NM_002243.3 (NON-CURRENT)
Match location:
Position 1332 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044611)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATTCTGAGAAACAGCAA
Hairpin Sequence:
5'-CCGG-GCAGATTCTGAGAAACAGCAA-CTCGAG-TTGCTGTTTCTCAGAATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATTCTGAGAAACAGCAACTCGAGTTGCTGTTTCTCAGAATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATTCTGAGAAACAGCAACTCGAGTTGCTGTTTCTCAGAATCTGC-3'

Other clones with same target sequence:

(none)