Construct: shRNA TRCN0000044614

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014139.1-5295s1c1
DNA Barcode:
GCCTAGATAGTATGAAAGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCN11A (11280)
Gene Description:
sodium voltage-gated channel alpha subunit 11
Transcript:
RefSeq NM_014139.1 (NON-CURRENT)
Match location:
Position 5295 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000044614)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAGATAGTATGAAAGCAA
Hairpin Sequence:
5'-CCGG-GCCTAGATAGTATGAAAGCAA-CTCGAG-TTGCTTTCATACTATCTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAGATAGTATGAAAGCAACTCGAGTTGCTTTCATACTATCTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAGATAGTATGAAAGCAACTCGAGTTGCTTTCATACTATCTAGGC-3'

Other clones with same target sequence:

(none)